Plot a BSA standard curve, fit a linear regression, and interpolate unknown protein concentrations -- the key practical skill tested in IBO 2018 and 2023.
Standard Data
#
[BSA] mg/mL
A595
Unknown Interpolation
Bradford assay: Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm upon binding protein.
The linear range is typically 0.1--0.5 mg/mL BSA. Points outside this range curve away from the line.