The full Yerevan bioinformatics practical, rebuilt to be worked through rather than read. Construct the signalling network, map expression onto the KEGG pathway, answer the written items, and check the alignment problems against the official key.
Problem 1.1.1: Construct chemokine signalling pathway (22 points)
Based on Appendix 1 sentences, add edges between nodes. Click a node, then another, choose type & state. Click on an edge to delete it. Submit to see your score.
💡 Instructions: Click a node (highlighted), then click another node → choose interaction type (Activation/Inhibition/Dissociation) and state (Direct/Indirect). Click on an edge line to delete it. Drag nodes to rearrange. Submit to check against official answer key.
Chemokine Signalling Pathway (KEGG view)
Gene expression & FC simulation (Q1.2.4, Q1.3.1, Q1.4.2-1.4.4)
Gene
Control expr
Case expr
FC (calculated)
Editable FC (for simulation)
PSF status: Not calculated yet. Click "Calculate PSF" to see signal values.
Q1.2.1 (8 points): True/False
Q1.2.2 (8 points): True/False
Q1.2.3 (8 points): True/False
Q1.3.2 (2 points): Which gene(s) have FC ≥ 2?
Q1.4.1 (4 points): Which process is suppressed?
Q1.4.2 (4 points): Gene whose mutation has least consequence
Q1.4.3 (4 points): Effect of Compound A (Akt inhibitor)
Q1.4.4 (8 points): Best compound to restore suppressed process