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Q35 - Classifying Crossover Events from Linear Tetrad Data

Theoretical A Real exam question - full text reproduced under IBO's CC BY-NC-SA 4.0 license

Centromere position can be mapped using linear tetrads in some fungi. If there is no cross-over between gene E and the centromere, four spores are arranged in a sequence of eeEE or EEee (Fig.Q.35A). If there is a cross-over between gene E and the centromere, four spores are arranged in a sequence of eEeE or EeEe (Fig.Q.35B). There are many types of crossovers involving 2, 3 and 4 strands of chromatids, as illustrated in Fig.Q.35C. Strain ab was crossed to strain a+ b+ and 100 linear tetrads were isolated. Those tetrads were divided into six classes as shown in the following table:

Class 1 Class 2 Class 3 Class 4 Class 5 Class 6
Spore 1 ab ab ab ab ab+ ab
Spore 2 a+b ab+ ab a+b+ a+b a+b+
Spore 3 ab+ a+b a+b+ ab ab+ ab+
Spore 4 a+b+ a+b+ a+b+ a+b+ a+b a+b
Count 15 29 47 2 2 5

Figure 1. Figure 1.

Using the information and data, determine which of the statements are true or which are false.

A. Locus a and b are located on the same arm of chromosome.
B. Class 4 involves a 4-strand double crossover.
C. Class 6 involves a 3-strand double crossover, with one between gene a and the centromere and between the centromere and gene b.
D. Class 5 involves a 2-strand double crossover.

Question reproduced from IBO 2016, Theoretical Paper A, licensed under CC BY-NC-SA 4.0 - attributed to the International Biology Olympiad. Open the full exam PDF · Community solutions (unofficial)