Q29 - Proteolytic Enzyme Substrate Specificity
Refer to the question paper for figures and tables.
a. Substrate 3 is cleaved most efficiently.
b. Alanine is the most preferred amino acid on either side of the cleavage site.
c. The enzyme most efficiently cleaves the peptide bond between a small hydrophobic amino acid residue and a large, aromatic hydrophobic amino acid residue.
d. A new substrate with a Km value of 0.3 would be more efficiently cleaved compared to the given substrates.