Q14 - Two-Step Enzymatic Release of Para-nitrophenol
The enzymatic reaction below includes two steps. para-nitrophenyl-peptide -> para-nitrophenol + peptide Para-nitrophenol has maximum absorbance at wavelength 405 nm. The progress curve of the reaction is shown below.
Figure 1.
Using the information and data, determine which of the statements are true or which are false.
A. The release of para-nitrophenol occurs during the first step of enzymatic mechanism.
B. The rate limiting step of this enzymatic mechanism is the second step.
C. Enzyme activity can be determined from the slope of region "a" of the curve.
D. One of the reaction products activates the enzyme.
Question reproduced from IBO 2018, Theoretical Paper 1, licensed under CC BY-NC-SA 4.0 - attributed to the International Biology Olympiad. Open the full exam PDF · Community solutions (unofficial)