Q16 - Restriction Enzyme Strategies for Cloning a Gene

Theoretical B Real exam question - full text reproduced under IBO's CC BY-NC-SA 4.0 license

Recombinant DNA & Biotechnology Techniques

A gene (coding sequence) can be expressed by cloning it into an expression plasmid using restriction enzymes and DNA-ligase. A plasmid (A), a gene of interest (B), and the recognition sequences for four restriction enzymes (C) are shown in the figure. Different cloning strategies, expressed in the statements below, could be used to insert the “Coding sequence and Terminator” of this gene into the plasmid to produce a recombinant plasmid that expresses the gene.

Using the information and data, determine which of the statements are true or which are false.

A. Digestion with SmaI followed by ligation can produce the desired recombinant plasmid.

B. Digestion with AatII and BamHI followed by ligation can produce the desired recombinant plasmid.

C. Digestion with BamHI + BglII followed by ligation can produce the desired recombinant plasmid.

D. The 'coding sequence' needs to be in-frame with the promoter

← Previous: Q15 Next: Q17 →

Question reproduced from IBO 2015, Theoretical Exam B, licensed under CC BY-NC-SA 4.0 - attributed to the International Biology Olympiad. Open the full exam PDF · Community solutions (unofficial)