Q14 - Matching Molecular Biology Techniques to Research Questions

Theoretical 1 Real exam question - full text reproduced under IBO's CC BY-NC-SA 4.0 license

Recombinant DNA & Biotechnology Techniques Biomolecular Analytical Techniques

With the advancement of DNA research, various technologies have been developed, and it has become important to select appropriate research methods according to one’s research purpose. Among the research methods M1 to M7, mark (T) if it is appropriate as the method that provides the most direct information on the following research purpose A - D, and mark (F) if it is inappropriate. Research methods (M1) DNA microarray (M2) Quantitative RT-PCR (M3) CRISPR-Cas9 method (M4) In situ hybridization (M5) Reproductive cloning (M6) Construction of iPS cell (M7) Metagenome analysis

Using the information and data, determine which of the statements are true or which are false.

A. To examine the site where a specific gene is expressed in a mouse tissue, it is appropriate to perform (M4).

B. To analyze the expression level of a specific gene in maple leaves, it is appropriate to perform (M2).

C. To search the Bacillus subtilis genome for genes the expression of which is induced when the nitrogen source is depleted. (M1)

D. To identify microbial species from microbial communities thriving in compost. (M7)

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Question reproduced from IBO 2020, Theoretical Exam 1, licensed under CC BY-NC-SA 4.0 - attributed to the International Biology Olympiad. Open the full exam PDF · Community solutions (unofficial)